Small RNA expression and miRNA modification dynamics in human oocytes and early embryos

Description

This collection of files contains small RNA-sequencing (sRNA-seq) data generated from human oocytes, zygotes, and pre-implantation embryos that were donated for research under an informed consent. Small non-coding RNAs (sRNAs) play important roles during the oocyte-to-embryo transition (OET), when the maternal phenotype is reprogrammed, and the embryo genome is gradually activated. sRNA sequencing libraries (n=27) were prepared from GV (n=6), Metaphase I (MI; n=3), and Metaphase II (MII; n=3) oocytes, zygotes (two-pronuclear zygote, 2PN, n=2; three-pronuclear zygote, 3PN, n=3), and 4- (n=5) and 8-cell (n=5) embryos using a low-input compatible library preparation method. The libraries were collected, prepared, and sequenced in two batches (batch I and batch II). We used sRNAbench (Aparicio-Puerta et al. 2019; Rueda et al. 2015) to map and annotate sRNAs that have been previously identified and in-house R scripts for downstream analyses. The provided files are in fastq.gz format with one file per sample. The dataset that supports these findings is accessible for scientific purposes. Due to patient consent and confidentiality agreements, data access will be evaluated by the Data Access Committee according to Finnish legislation. Data can be accessed internationally with valid Data Access Agreement, but data processing is restricted to Finland. This dataset is affiliated to a Data Access Committee (DAC), which is the body responsible for all data access decisions. You can apply for access by selecting the dataset name from https://sd-apply.csc.fi/catalogue (applying for access requires log in). For more information write to servicedesk@csc.fi (email subject SD Apply /Sensitive Data).
Show more

Year of publication

2021

Type of data

Authors

Competence Centre for Health Technologies (Tartu)

Kaarel Krjutškov - Contributor

Priit Paluoja - Contributor

Department of Biosciences and Nutrition

Juha Kere - Contributor

Helsinki University Hospital

Department of Obstetrics and Gynecology

Christel Hydén-Granskog - Creator

Dawit A. Yohannes - Contributor

Juha S. Tapanainen - Creator

Sanna Vuoristo - Creator

Timo Tuuri - Creator

Pauliina Paloviita Orcid -palvelun logo - Creator

University of Tartu

Estonian Genome Center

Urmo Võsa - Contributor

Project

Other information

Fields of science

Biochemistry, cell and molecular biology; Genetics, developmental biology, physiology

Language

English

Open access

Restricted access

License

License Not Specified

Keywords

embryo, embryo genome activation, isomiR, microRNA, oocyte, oocyte short piRNA, oocyte-to-embryo transition, zygote, small RNA

Subject headings

Temporal coverage

undefined

Related to this research data